Browsing Magister Scientiae - MSc (Biotechnology) by Author "den Haan, Riaan"
Now showing items 1-5 of 5
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Comparison of multi-gene integration strategies in CRISPR-based transformation of Saccharomyces cerevisiae
Jacob, Odwa (University of Western Cape, 2021)Saccharomyces cerevisiae is an important host in industrial biotechnology. This yeast is the host of choice for the first and second-generation biofuels for ethanol production. Genome modification in S. cerevisiae has been ... -
The effect of blocking selected endocytic mechanisms on heterologous protein secretion in the yeast saccharomyces cerevisiae
Freeman, Kim (University of the Western Cape, 2018)The yeast Saccharomyces cerevisiae is considered a good host used for heterologous protein production due to the organism’s microbial safety, rapid growth and eukaryotic post- translational processing. As a fermentative ... -
The in vitro detection and measurement of the unfolded protein response in Saccharomyces cerevisiae
Cedras, Gillian (University of the Western Cape, 2018)Bioethanol is currently the most widely used biofuel and can be used as a direct replacement for current fossil fuel based transportation fuels. Consolidated bioprocessing (CBP), in which bioethanol is produced in a single ... -
Using CRISPR/Cas9 to construct consolidated bioprocessing strains from natural isolates of Saccharomyces cerevisiae
Minnaar, Letitia (University of the Western Cape, 2022)Saccharomyces cerevisiae has gained much attention as a host for cellulosic bioethanol production using consolidated bioprocessing (CBP) methodologies, due to its high ethanol producing titres, heterologous protein producing ... -
Valorisation of brewers’ spent grain: enzymatic hydrolysis in the production of xylooligosaccharides
Arries, Chelsey (University of the Western Cape, 2023)The beverage industry constitutes approximately 26% of all food wastes, making it one of the largest contributors in this waste segment. By utilising waste or by-products from agriculture and food production in manufacturing ...